NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 2,969.2
Mean read quality 21.9
Median read length 3,790.0
Median read quality 21.2
Number of reads 28,549.0
Read length N50 3,870.0
STDEV read length 1,318.9
Total bases 84,768,358.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 28549 (100.0%) 84.8Mb
>Q7 28549 (100.0%) 84.8Mb
>Q10 27583 (96.6%) 82.0Mb
>Q12 25638 (89.8%) 75.8Mb
>Q15 22521 (78.9%) 66.2Mb
Top 5 highest mean basecall quality scores and their read lengths
1 42.2 (37)
2 42.0 (473)
3 42.0 (543)
4 42.0 (576)
5 42.0 (577)
Top 5 longest reads and their mean basecall quality score
1 9258 (17.7)
2 8713 (13.3)
3 8427 (17.4)
4 8402 (12.4)
5 8394 (14.7)

Summary statistics after filtering

General summary
Mean read length 2,969.3
Mean read quality 21.9
Median read length 3,790.0
Median read quality 21.2
Number of reads 28,548.0
Read length N50 3,870.0
STDEV read length 1,318.8
Total bases 84,768,339.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 28548 (100.0%) 84.8Mb
>Q7 28548 (100.0%) 84.8Mb
>Q10 27582 (96.6%) 82.0Mb
>Q12 25637 (89.8%) 75.8Mb
>Q15 22520 (78.9%) 66.2Mb
Top 5 highest mean basecall quality scores and their read lengths
1 42.2 (37)
2 42.0 (473)
3 42.0 (543)
4 42.0 (576)
5 42.0 (577)
Top 5 longest reads and their mean basecall quality score
1 9258 (17.7)
2 8713 (13.3)
3 8427 (17.4)
4 8402 (12.4)
5 8394 (14.7)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality plot using a kernel density estimation