MIrROR – Microbial Identification using rRNA Operon Region

Project - PRJNA383904

MinION ribosomal RNA profiling

Descriptions

For this bio project, we profiled entire ribosomal operons from bacteria within our complex samples. These rRNA operons are amplified using domain-specific forward primers in the small ribosomal RNA subunit (16S), domain-specific reverse primers within the large rRNA subunit (23S), and a High Fidelity Taq polymerase. This rRNA operon profiling approach yields an amplicon containing both 16S/23S ribosomal subunits for phylogenetic assignment (4200 bp of sequence), plus the ITS region (500+ bp) containing species/strain information to distinguish various members within the microbial community.

External Links

ENA website

NCBI website

Publication

Profiling bacterial communities by MinION sequencing of ribosomal operons
Lee J. Kerkhof, et al. 2017

Runs 24

Sample Accession	Run Accession	Scientific Name	Instrument Platform	Instrument Model	Library Name
SAMN06819349	SRR5498689	soil metagenome	Oxford Nanopore	MinION	Oprun1A_BC06
SAMN06819350	SRR5498688	soil metagenome	Oxford Nanopore	MinION	Oprun1B_BC06
SAMN06819351	SRR5498687	soil metagenome	Oxford Nanopore	MinION	Oprun2A_BC11
SAMN06819352	SRR5498686	soil metagenome	Oxford Nanopore	MinION	Oprun2B_BC11
SAMN06819353	SRR5498685	soil metagenome	Oxford Nanopore	MinION	Oprun1A_BC05
SAMN06819354	SRR5498684	soil metagenome	Oxford Nanopore	MinION	Oprun1B_BC05
SAMN06819355	SRR5498683	soil metagenome	Oxford Nanopore	MinION	Oprun2A_BC10
SAMN06819356	SRR5498682	soil metagenome	Oxford Nanopore	MinION	Oprun2B_BC10
SAMN06819357	SRR5498681	soil metagenome	Oxford Nanopore	MinION	Oprun1A_BC04
SAMN06819358	SRR5498680	soil metagenome	Oxford Nanopore	MinION	Oprun1B_BC04
SAMN06819359	SRR5498679	soil metagenome	Oxford Nanopore	MinION	Oprun2A_BC09
SAMN06819360	SRR5498678	soil metagenome	Oxford Nanopore	MinION	Oprun2B_BC09
SAMN06819361	SRR5498677	soil metagenome	Oxford Nanopore	MinION	Oprun1A_BC03
SAMN06819362	SRR5498676	soil metagenome	Oxford Nanopore	MinION	Oprun1B_BC03
SAMN06819363	SRR5498675	soil metagenome	Oxford Nanopore	MinION	Oprun2A_BC08
SAMN06819364	SRR5498674	soil metagenome	Oxford Nanopore	MinION	Oprun2B_BC08
SAMN06819365	SRR5498673	soil metagenome	Oxford Nanopore	MinION	Oprun1A_BC12
SAMN06819366	SRR5498672	soil metagenome	Oxford Nanopore	MinION	Oprun1B_BC12
SAMN06819367	SRR5498671	soil metagenome	Oxford Nanopore	MinION	Oprun2A_BC02
SAMN06819368	SRR5498670	soil metagenome	Oxford Nanopore	MinION	Oprun2B_BC02
SAMN06819369	SRR5498669	soil metagenome	Oxford Nanopore	MinION	Oprun1A_BC01
SAMN06819370	SRR5498668	soil metagenome	Oxford Nanopore	MinION	Oprun1B_BC01
SAMN06819371	SRR5498667	soil metagenome	Oxford Nanopore	MinION	Oprun2A_BC07
SAMN06819372	SRR5498666	soil metagenome	Oxford Nanopore	MinION	Oprun2B_BC07